Abstract - What is qPCR (quantitative real-time polymerase chain reaction), and how does it work? What is the chemistry behind this powerful technique? This presentation will discuss the chemistry and biochemistry that makes it work. This seminar reviews associated applications when working with DNA, RNA, and protein analysis. DNA applications include mutation detection, single nucleotide polymorphisms, and high resolution melt. RNA applications reviewed are gene expression and small RNA. The final section of the talk examines protein expression and digital PCR.
Biography - Geoff Jackson is a Regional Field Application Scientist for Thermo Fisher Scientific with over 30 years of experience in molecular biology and pathogen detection.
Prior to this role, Geoff was a lead biologist and program manager at Centers of Disease Control and Prevention (CDC) for almost 10 years. He is fluent in many scientific and molecular biology techniques that include but are not limited to basic science benchwork; pathogen detection assay development; bioterrorism response and prevention; program management; and emergency management.
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